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Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a dual inhibitor of the matrix metalloproteinases (MMPs) and some adamalysins, two families of extracellular and cell surface metalloproteinases that function in extracellular matrix turnover and the shedding of cell surface proteins. The mechanism of inhibition of MMPs by TIMPs has been well characterized, and since the catalytic domains of MMPs and adamalysins are homologous, it was assumed that the interaction of TIMP-3 with adamalysins is closely similar. Here we report that the inhibition of the extracellular region of ADAM-17 (tumor necrosis factor alpha-converting enzyme (TACE)) by the inhibitory domain of TIMP-3 (N-TIMP-3) shows positive cooperativity. Also, mutations in the core of the MMP interaction surface of N-TIMP-3 dramatically reduce the binding affinity for MMPs but have little effect on the inhibitory activity for TACE. These results suggest that the mechanism of inhibition of ADAM-17 by TIMP-3 may be distinct from that for MMPs. The mutant proteins are also effective inhibitors of tumor necrosis factor alpha (TNF-alpha) release from phorbol ester-stimulated cells, indicating that they provide a lead for engineering TACE-specific inhibitors that may reduce side effects arising from MMP inhibition and are possibly useful for treatment of diseases associated with excessive TNF-alpha levels such as rheumatoid arthritis.

Original publication

DOI

10.1074/jbc.C500220200

Type

Journal article

Journal

J Biol Chem

Publication Date

23/09/2005

Volume

280

Pages

32877 - 32882

Keywords

ADAM Proteins, ADAM17 Protein, Binding Sites, Catalysis, Catalytic Domain, Cell Membrane, Dose-Response Relationship, Drug, Enzyme Inhibitors, Humans, Kinetics, Matrix Metalloproteinase Inhibitors, Matrix Metalloproteinases, Models, Molecular, Mutation, Phorbol Esters, Plasmids, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Tissue Inhibitor of Metalloproteinase-3, Tumor Necrosis Factor-alpha