Zymography is an electrophoretic technique enabling visualization of the number and approximate size of peptidases in a sample on the basis of their hydrolysis of a protein substrate within the gel. The technique is particularly useful for analyzing the peptidase composition of complex biological samples because visualization depends directly on proteolytic activity. This unit presents a representative zymography protocol for the study of matrix metallopeptidases (MMPs).
Unit - 21.15
Electrophoresis, Polyacrylamide Gel, Hydrolysis, Matrix Metalloproteinases, Molecular Weight, Substrate Specificity